C6orf141 Polyclonal antibody

C6orf141 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24355-1-AP

Synonyms

C6orf141

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Validation Data Gallery

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  WB analysis of A431 using 24355-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24355-1-AP (C6orf141 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24355-1-AP (C6orf141 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24355-1-AP (C6orf141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24355-1-AP targets C6orf141 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C6orf141 fusion protein Ag19668
• Full Name: chromosome 6 open reading frame 141
• Calculated Molecular Weight: 244 aa, 27 kDa
• Observed Molecular Weight: 27-31 kDa
• GenBank Accession Number: BC036917
• Gene Symbol: C6orf141
• Gene ID (NCBI): 135398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.