SUGCT Polyclonal antibody

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• SUGCT Polyclonal antibody (21589-1-AP)

SUGCT Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21589-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C7orf10, Dermal papilla-derived protein 13, DERP13, EC:2.8.3.13, ORF19

• 

  WB analysis using 21589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 21589-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 21589-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 21589-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 21589-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 21589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse pancreas tissue, mouse skeletal muscle tissue, HepG2 cells, HEK-293 cells, rat kidney tissue
• Positive IHC detected in: human kidney tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21589-1-AP targets SUGCT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16159

  Product name: Recombinant human C7orf10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 123-471 aa of BC098318

  Sequence: VKIIKELAAVCDVFVENYVPGKLSAMGLGYEDIDEIAPHIIYCSITGYGQTGPISQRAGYDAVASAVSGLMHITGPENGDPVRPGVAMTDLATGLYAYGAIMAGLIQKYKTGKGLFIDCNLLSSQVACLSHIAANYLIGQKEAKRWGTAHGSIVPYQAFKTKDGYIVVGAGNNQQFATVCKILDLPELIDNSKYKTNHLRVHNRKELIKILSERFEEELTSKWLYLFEGSGVPYGPINNMKNVFAEPQNAVSGFQSLLHSLAHGPFLHLQGSARVLHNGLVMEMEHPTVGKISVPGPAVRYSKFKMSEARPPPLLGQHTTHILKEVLRYDDRAIGELLSAGVVDQHETH

• Full Name: chromosome 7 open reading frame 10
• Calculated Molecular Weight: 471 aa, 51 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC098318
• Gene Symbol: C7orf10
• Gene ID (NCBI): 79783
• RRID: AB_10734435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAC7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf10 is also named as SUGCT, DERP13 and belongs to the CaiB/BaiF CoA-transferase family. It may function as part of a multiunit enzyme complex (A), similar to the alpha-ketoglutarate dehydrogenase system, or as the second of two independent enzymes, the first of which decarboxylates alpha-ketoadipate to glutarate (B). This protein has 4 isoforms produced by alternative splicing with the molecular weight from 43 kDa to 51 kDa.

Publications

What published studies show

SUGCT Polyclonal antibody (Cat# 21589-1-AP) has 5 total citations. These appear in Cell Mol Life Sci, Front Aging Neurosci, FASEB J, Transl Androl Urol, and bioRxiv. The associated research fields include metabolic syndrome and diabetes, neurodegenerative disease (ALS), organic acid metabolism (glutaric aciduria type 1), and acute kidney injury. All citations used the antibody for Western blot, with one also employing IHC.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Mol Life Sci; Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype.; Authors - Joanna Niska-Blakie
human	WB	Front Aging Neurosci; A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations; Authors - Scott P Allen
mouse	WB	FASEB J; Odd-Chain Dicarboxylic Acid Feeding Produces a Glutaric Aciduria Type 1-Like Metabolic Signature in Mice.; Authors - Adam C Richert
mouse	WB,IHC	Transl Androl Urol; Identification of core genes in acute kidney injury: evidence from multi-omics human transcriptomic data and in vivo models; Authors - Pengxiao Sun
mouse	WB	bioRxiv; Odd-chain dicarboxylic acid feeding recapitulates the biochemical phenotype of glutaric aciduria type 1 in mice; Authors - Adam C Richert