Tested Applications
| Positive WB detected in | NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22159-1-AP targets CCZ1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17804 Product name: Recombinant human C7orf28A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 133-482 aa of BC132749 Sequence: VYSSVLRQCYSMYKLFNGTFLKAMEDGGVKLLKERLEKFFHRYLQTLHLQSCDLLDIFGGISFFPLDKMTYLKIQSFINRMEESLNIVKYTAFLYNDQLIWSGLEQDDMRILYKYLTTSLFPRHIEPELAGRDSPIRAEMPGNLQHYGRFLTGPLNLNDPDAKCRFPKIFVNTDDTYEELHLIVYKAMSAAVCFMIDASVHPTLDFCRRLDSIVGPQLTVLASDICEQFNINKRMSGSEKEPQFKFIYFNHMNLAEKSTVHMRKTPSVSLTSVHPDLMKILGDINSDFTRVDEDEEIIVKAMSDYWVVGKKSDRRELYVILNQKNANLIEVNEEVKKLCATQFNNIFFLD Predict reactive species |
| Full Name | chromosome 7 open reading frame 28A |
| Calculated Molecular Weight | 482 aa, 56 kDa |
| Observed Molecular Weight | 50-56 kDa |
| GenBank Accession Number | BC132749 |
| Gene Symbol | CCZ1 |
| Gene ID (NCBI) | 51622 |
| RRID | AB_2879011 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P86790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCZ1 antibody 22159-1-AP | Download protocol |
| IP protocol for CCZ1 antibody 22159-1-AP | Download protocol |
| WB protocol for CCZ1 antibody 22159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCZ1 Polyclonal antibody (catalog number 22159-1-AP) has 5 total citations. These appear in Autophagy, Nature Communications, Journal of Neuroinflammation, Biomedicines, and FASEB Journal. The associated research fields include autophagy and autophagosome maturation, endosomal trafficking and lipid homeostasis, neuroinflammation and oxidative stress, cervical squamous cell carcinoma progression, and phagolysosome formation in neutrophils and macrophages.
| Species | Application | Title |
|---|---|---|
Autophagy GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes | ||
Nat Commun Endosomal trafficking participates in lipid droplet catabolism to maintain lipid homeostasis
| ||
J Neuroinflammation Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH. | ||
Biomedicines CCZ1 Accelerates the Progression of Cervical Squamous Cell Carcinoma by Promoting MMP2/MMP17 Expression
| ||
FASEB J ATP7A Maintains Bactericidal Function of Neutrophils and Macrophages via Regulating the Formation and Activation of Phagolysosomes |
