C7orf43 Polyclonal antibody

C7orf43 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27790-1-AP

Synonyms

C7orf43

---

Validation Data Gallery

• 

  WB analysis using 27790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27790-1-AP (C7orf43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27790-1-AP targets C7orf43 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C7orf43 fusion protein Ag27149
• Full Name: chromosome 7 open reading frame 43
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC015722
• Gene Symbol: C7orf43
• Gene ID (NCBI): 55262
• RRID: AB_2880972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The predicted MW of C7orf43 is 62 kDa. Catalog#27790-1-AP recognises 65-70 kDa band may due to phosphorylation.