C7orf58 Polyclonal antibody

C7orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20924-1-AP

Synonyms

UNQ9432/PRO34713, CPED1, Cadherin-like and PC-esterase domain-containing protein 1

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Validation Data Gallery

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  WB analysis of A375 using 20924-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20924-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20924-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20924-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20924-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20924-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20924-1-AP (C7orf58 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach using 20924-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20924-1-AP (C7orf58 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A375 using 20924-1-AP

  Immunofluorescent analysis of A375 cells using 20924-1-AP (C7orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A375 using 20924-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using C7orf58 antibody (20924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A375 cells, A431 cells, HEK-293 cells, HeLa cells, L02 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20924-1-AP targets C7orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C7orf58 fusion protein Ag15193
• Full Name: chromosome 7 open reading frame 58
• Calculated Molecular Weight: 1026 aa, 117 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC030538
• Gene Symbol: C7orf58
• Gene ID (NCBI): 79974
• RRID: AB_10697813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis; Authors - Siwei Pan