C8G Polyclonal antibody

C8G Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27200-1-AP

Synonyms

C8C, C8G

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Validation Data Gallery

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  WB analysis using 27200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27200-1-AP (C8G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27200-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27200-1-AP (C8G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human milk, human placenta tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27200-1-AP targets C8G in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C8G fusion protein Ag17949
• Full Name: complement component 8, gamma polypeptide
• Calculated Molecular Weight: 202 aa, 22 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC113626
• Gene Symbol: C8G
• Gene ID (NCBI): 733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gamma chain of complement component 8 (C8G) is one of three different subunits (alpha, beta, and gamma) of the eighth complement component (C8), which is a component of the MAC (membrane attack complex) (PMID:33382892). Although the roles of C8A and C8B are directly associated with MAC formation, C8G is not essential in the assembly and activity of the MAC (PMID:11960635). Moreover, C8G was shown to inhibit neuroinflammation by interfering with the sphingosine-1-phosphate (S1P)-S1PR2 interaction (PMID:34149451).