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C9 Polyclonal antibody

C9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25891-1-AP

Synonyms

C9, complement component 9, Complement component C9

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C9 Polyclonal antibody (25891-1-AP)

    WB analysis of human plasma using 25891-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 25891-1-AP (C9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25891-1-AP targets C9 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9 fusion protein Ag23137
Full Name complement component 9
Calculated Molecular Weight 559 aa, 63 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC020721
Gene Symbol C9
Gene ID (NCBI) 735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The final consequence of complement activation is the formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. The MAC plays a critical role in complement-mediated hemolysis and inflammasome activation (PMID: 27444648). Complement component 9 (C9) is 1 of 5 late-acting proteins of complement that participate in the assembly and function of the MAC. C9 may be cleaved by thrombin to generate fragments C9a (25 kDa) and C9b (37 kDa) (PMID: 7523406).

Protocols

Product Specific Protocols
WB protocol for C9 antibody 25891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols