ERCC6L2 Polyclonal antibody

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  WB analysis of PC-3 using 27028-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27028-1-AP (ERCC6L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27028-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27028-1-AP (ERCC6L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 27028-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27028-1-AP (ERCC6L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ERCC6L2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27028-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

DNA excision repair protein ERCC-6-like 2, DNA repair and recombination protein RAD26-like, EC:3.6.4.-, RAD26L, SR278

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Tested Applications

• Positive WB detected in: PC-3 cells, HepG2 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27028-1-AP targets ERCC6L2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19348

  Product name: Recombinant human C9orf102 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 358-712 aa of BC140702

  Sequence: KRELATGRKAMQRLAKKMSGWFLRRTKTLIKDQLPKKEDRMVYCSLTDFQKAVYQTVLETEDVTLILQSSEPCTCRSGQKRRNCCYKTNSHGETVKTLYLSYLTVLQKVANHVALLQAASTSKQQETLIKRICDQVFSRFPDFVQKSKDAAFETLSDPKYSGKMKVLQQLLNHCRKNRDKVLLFSFSTKLLDVLQQYCMASGLDYRRLDGSTKSEERLKIVKEFNSTQDVNICLVSTMAGGLGLNFVGANVVVLFDPTWNPANDLQAIDRAYRIGQCRDVKVLRLISLGTVEEIMYLRQIYKQQLHCVVVGSENAKRYFEAVQGSKEHQGELFGIHNLFKFRSQGSCLTKDILEV

• Full Name: chromosome 9 open reading frame 102
• Calculated Molecular Weight: 712 aa, 81 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC140702
• Gene Symbol: ERCC6L2
• Gene ID (NCBI): 375748
• RRID: AB_2880729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T890
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERCC6L2 , also known as RAD26L or C9orf102 belongs to the SNF2/RAD54 helicase family and is expressed in bone marrow. C9orf102 may be involved in early DNA damage response. C9orf102 is phosphorylated by NEK6. C9orf102 exists some isoforms with the molecular weight 177, 80, 60, 40 kDa.