AFAF/EQTN Polyclonal antibody

AFAF/EQTN Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23168-1-AP

Synonyms

AFAF, C9orf11, EQTN, Equatorin

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Validation Data Gallery

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  WB analysis of HepG2 using 23168-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23168-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 23168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFAF/EQTN antibody (23168-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23168-1-AP targets AFAF/EQTN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFAF/EQTN fusion protein Ag19656
• Full Name: chromosome 9 open reading frame 11
• Calculated Molecular Weight: 294 aa, 33 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC137351
• Gene Symbol: AFAF
• Gene ID (NCBI): 54586
• RRID: AB_2879222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The AFAF gene (also known as EQTN and C9orf11) encodes a membrane protein that is specifically localized to the acrosomal membrane. AFAF is involved in the process of fertilization and acrosome biogenesis. Expression analysis demonstrates that AFAF is highly expressed in the testis, although minor expression was seen in other tissues. AFAF has 3 isoforms with molecular weights of 14, 29, and 33 kDa. However, one article detected molecular weight between 40-65 kDa in mouse testis (PMID: 19605790).