NGX6 Polyclonal antibody

NGX6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21593-1-AP

Synonyms

TMEM8B, C9orf127, Transmembrane protein 8B, Protein NAG-5, Nasopharyngeal carcinoma-associated gene 6 protein

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Validation Data Gallery

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  IHC staining of rat small intestine using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse intestine using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21593-1-AP (NGX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21593-1-AP (NGX6 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21593-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21593-1-AP (NGX6 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, mouse intestine, rat small intestine tissue, rat stomach tissue, mouse stomach tissue, mouse testis tissue, human cervical cancer tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21593-1-AP targets NGX6 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGX6 fusion protein Ag16167
• Full Name: chromosome 9 open reading frame 127
• Calculated Molecular Weight: 472 aa, 52 kDa
• GenBank Accession Number: BC098296
• Gene Symbol: NGX6
• Gene ID (NCBI): 51754
• RRID: AB_2878889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGX6 (also known as TMEM8B or C9orf127) is a transmembrane protein containing an epidermal growth factor (EGF)-like domain. It may function as a regulator of the EGFR pathway. NGX6 has been reported as a tumor suppressor which may function in cell growth, proliferation and adhesion (PMID: 19755717; 12918109; 20705583).