C9orf171 Polyclonal antibody

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  WB analysis using 24969-1-AP

  Mouse testis tissue lysates were subjected to SDS PAGE followed by western blot with 24969-1-AP (C9orf171 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 24969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 24969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 24969-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C9orf171 antibody (24969-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C9orf171 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Cat No. 24969-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

Cilia- and flagella-associated protein 77, CFAP77

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24969-1-AP targets C9orf171 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21797

  Product name: Recombinant human C9orf171 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-284 aa of BC118593

  Sequence: MPEARSSGPDLTRWRKQQQPVRRTVSQVCPPPRRPLTVADIRSGMENERLGVVRDSMFQNPLIVKAELGKPRERSYSLPGINFNYGLYIRGLDGGVPEAIGRWNVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENLLYRQLNDIRISDQDDRRMKKEPPPLPPNMTFGIRARPSTPFFDLLQHRYLQLWVQEQKATQKAIKLEKKQKVVLGKLYETRSSQLRKYKPPVKLDTLWHMPHFQKVGRHLDTFPTEADRQRALKAHREECAVRQGTLRMGNYTHP

• Full Name: chromosome 9 open reading frame 171
• Calculated Molecular Weight: 320 aa, 36 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC118593
• Gene Symbol: C9orf171
• Gene ID (NCBI): 389799
• RRID: AB_3085763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZQR2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.