C9orf171 Polyclonal antibody

C9orf171 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24969-1-AP

Synonyms

C9orf171

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Validation Data Gallery

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  WB analysis using 24969-1-AP

  Mouse testis tissue lysates were subjected to SDS PAGE followed by western blot with 24969-1-AP (C9orf171 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 24969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 24969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-251 using 24969-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C9orf171 antibody (24969-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IF detected in: mouse testis tissue, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24969-1-AP targets C9orf171 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C9orf171 fusion protein Ag21797
• Full Name: chromosome 9 open reading frame 171
• Calculated Molecular Weight: 320 aa, 36 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC118593
• Gene Symbol: C9orf171
• Gene ID (NCBI): 389799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.