C9orf41/CARNMT1 Polyclonal antibody

• 

  WB analysis of HepG2 using 24068-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24068-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 24068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf41/CARNMT1 antibody (24068-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

C9orf41/CARNMT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24068-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

C9orf41, CARNMT1, Carnosine N-methyltransferase, EC:2.1.1.22

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

24068-1-AP targets C9orf41/CARNMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21268

  Product name: Recombinant human C9orf41 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 61-409 aa of BC034033

  Sequence: LEREHFWKIINAFRYYGTSMHERVNRTERQFRSLPANQQKLLPQFLLHLDKIRKCIDHNQEILLTIVNDCIHMFENKEYGEDGNGKIMPASTFDMDKLKSTLKQFVRDWSETGKAERDACYQPIIKEILKNFPKERWDPSKVNILVPGAGLGRLAWEIAMLGYACQGNEWSFFMLFSSNFVLNRCSEINKYKLYPWIHQFSNNRRSADQIRPIFFPDVDPHSLPPGSNFSMTAGDFQEIYSECNTWDCIATCFFIDTAHNVIDYIDTIWKILKPGGIWINLGPLLYHFENLANELSIELSYEDIKNVVLQYGFKVEVEKESVLSTYTVNDLSMMKYYYECVLFVVRKPQ

• Full Name: chromosome 9 open reading frame 41
• Calculated Molecular Weight: 409 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC034033
• Gene Symbol: C9orf41
• Gene ID (NCBI): 138199
• RRID: AB_2879425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8N4J0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The enzyme CARNMT1 (carnosine N-methyltransferase 1, also known as C9orf41), conserved from yeast to human, was identified as an N1 position-specific methyltransferase that catalyzes the conversion of carnosine to anserine (β-alanyl-N-π-methyl-L-histidine).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Genes Dev; Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism; Authors - Tadahiro Shimazu ; KO Validated