C9orf41/CARNMT1 Polyclonal antibody

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C9orf41/CARNMT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24068-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

C9orf41, CARNMT1, Carnosine N-methyltransferase, EC:2.1.1.22

• 

  WB analysis of HepG2 using 24068-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 24068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 24068-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 24068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf41/CARNMT1 antibody (24068-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24068-1-AP targets C9orf41/CARNMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21268

  Product name: Recombinant human C9orf41 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 61-409 aa of BC034033

  Sequence: LEREHFWKIINAFRYYGTSMHERVNRTERQFRSLPANQQKLLPQFLLHLDKIRKCIDHNQEILLTIVNDCIHMFENKEYGEDGNGKIMPASTFDMDKLKSTLKQFVRDWSETGKAERDACYQPIIKEILKNFPKERWDPSKVNILVPGAGLGRLAWEIAMLGYACQGNEWSFFMLFSSNFVLNRCSEINKYKLYPWIHQFSNNRRSADQIRPIFFPDVDPHSLPPGSNFSMTAGDFQEIYSECNTWDCIATCFFIDTAHNVIDYIDTIWKILKPGGIWINLGPLLYHFENLANELSIELSYEDIKNVVLQYGFKVEVEKESVLSTYTVNDLSMMKYYYECVLFVVRKPQ

• Full Name: chromosome 9 open reading frame 41
• Calculated Molecular Weight: 409 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC034033
• Gene Symbol: C9orf41
• Gene ID (NCBI): 138199
• RRID: AB_2879425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8N4J0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The enzyme CARNMT1 (carnosine N-methyltransferase 1, also known as C9orf41), conserved from yeast to human, was identified as an N1 position-specific methyltransferase that catalyzes the conversion of carnosine to anserine (β-alanyl-N-π-methyl-L-histidine).

Publications

What published studies show

The C9orf41/CARNMT1 Polyclonal antibody (Cat# 24068-1-AP) has 1 citation. It was used in a study published in Genes & Development (2023), where it was applied by Western blot in mouse samples to investigate how CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism. The research falls within the fields of epigenetic regulation, histidine methylation, and RNA metabolism.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Genes Dev; Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism; Authors - Tadahiro Shimazu ; KO Validated