C9orf61 Polyclonal antibody

C9orf61 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 25630-1-AP

Synonyms

C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 25630-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25630-1-AP (C9orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 25630-1-AP is C9orf61 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C9orf61 fusion protein Ag22242
• Full Name: chromosome 9 open reading frame 61
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC113685
• Gene Symbol: C9orf61
• Gene ID (NCBI): 9413
• RRID: AB_2880170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB,IHC,CoIP	Tissue Cell; FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins; Authors - Jiakun Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chrisovalantou (Verified Customer) (10-31-2024)

Antibody unfortunately could not reliably and specifically detect the protein of interest. Many unspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y, HEK293T

C9orf61 Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y, HEK293T (Cat no:25630-1-AP)