C9orf64 Polyclonal antibody

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  WB analysis of HeLa using 27797-1-AP

  WB result of C9orf64 antibody (27797-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C9orf64 transfected HeLa cells.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27797-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27797-1-AP (C9orf64 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

C9orf64 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27797-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Queuosine-nucleotide N-glycosylase/hydrolase, Queuosine 5'-phosphate N-glycosylase/hydrolase, Queuine salvage protein QNG1, Q-nucleotide N-glycosylase 1, QNG1

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27797-1-AP targets C9orf64 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23069

  Product name: Recombinant human C9orf64 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-341 aa of BC004407

  Sequence: MDGLLNPRESSKFIAENSRDVFIDSGGVRRVAELLLAKAAGPELRVEGWKALHELNPRAADEAAVNWVFVTDTLNFSFWSEQDEHKCVVRYRGKTYSGYWSLCAAVNRALDEGIPITSASYYATVTLDQVRNILRSDTDVSMPLVEERHRILNETGKILLEKFGGSFLNCVRESENSAQKLMHLVVESFPSYRDVTLFEGKRVSFYKRAQILVADTWSVLEGKGDGCFKDISSITMFADYRLPQVLAHLGALKYSDDLLKKLLKGEMLSYGDRQEVEIRGCSLWCVELIRDCLLELIEQKGEKPNGEINSILLDYYLWDYAHDHREDMKGIPFHRIRCIYY

• Full Name: chromosome 9 open reading frame 64
• Calculated Molecular Weight: 341 aa, 39 kDa
• Observed Molecular Weight: 34-39 kDa
• GenBank Accession Number: BC004407
• Gene Symbol: C9orf64
• Gene ID (NCBI): 84267
• RRID: AB_2918132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T6V5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.