C9orf78 Polyclonal antibody

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  WB analysis using 14496-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14496-1-AP (C9orf78 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14496-1-AP

  IP result of anti-C9orf78 (IP:14496-1-AP, 4ug; Detection:14496-1-AP 1:500) with HepG2 cells lysate 2200 ug.

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  IHC staining of human stomach cancer using 14496-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14496-1-AP (C9orf78 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bladder using 14496-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 14496-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14496-1-AP

  Immunofluorescent analysis of HepG2 cells using 14496-1-AP (C9orf78 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14496-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (14496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

C9orf78 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14496-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach cancer tissue, human bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

14496-1-AP targets C9orf78 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5919

  Product name: Recombinant human C9orf78 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-289 aa of BC007664

  Sequence: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY

• Full Name: chromosome 9 open reading frame 78
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007664
• Gene Symbol: C9orf78
• Gene ID (NCBI): 51759
• RRID: AB_10644338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZ63
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,CoIP,ChIP	Sci Adv; FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production; Authors - Weilong Chen