SPACA9 Polyclonal antibody

SPACA9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26034-1-AP

Synonyms

C9orf9, SPACA9, Uncharacterized protein C9orf9

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Validation Data Gallery

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  WB analysis of mouse testis using 26034-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 26034-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 26034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 26034-1-AP

  Immunofluorescent analysis of A431 cells using 26034-1-AP (C9orf9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse liver tissue, rat testis tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26034-1-AP targets SPACA9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPACA9 fusion protein Ag23320
• Full Name: chromosome 9 open reading frame 9
• Observed Molecular Weight: 18 kDa, 30 kDa
• GenBank Accession Number: BC012940
• Gene Symbol: SPACA9
• Gene ID (NCBI): 11092
• RRID: AB_2880345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C9orf9 remains largely unknown. Catalog#26034-1-AP is a rabbit polyclonal antibody raised against the full-length of human C9orf9. Two isoforms were produced by alternative splicing with predicted MW of 19 and 26 kDa (UniProt). Two bands at 16 kDa and 30 kDa could be detected using the present rabbit polyclonal antibody 26034-1-AP.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma