CA1 Polyclonal antibody

CA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13198-2-AP


CA I, CA1, CAB, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, carbonic anhydrase I

Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, rat spleen tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13198-2-AP targets CA1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA1 fusion protein Ag3932
Full Name carbonic anhydrase I
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC027890
Gene Symbol CA1
Gene ID (NCBI) 759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).


Product Specific Protocols
WB protocol for CA1 antibody 13198-2-APDownload protocol
IHC protocol for CA1 antibody 13198-2-APDownload protocol
IP protocol for CA1 antibody 13198-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Acta Neuropathol Commun

Upregulation of carbonic anhydrase 1 beneficial for depressive disorder

Authors - Ke Cheng
  • KO Validated

J Ginseng Res

Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway

Authors - Mei-Li Lu


Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Authors - Brittany R Jenkins

J Inflamm Res

M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

Authors - Xianqin Song

Am J Physiol Lung Cell Mol Physiol

Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.

Authors - Kathleen Boyne

Front Pharmacol

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.

Authors - Meili Lu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Brittany (Verified Customer) (09-27-2019)

Using the CA-1 primary antibody with mouse colon scrapes yielded very clean bands with western blotting. Immunofluorescence also gave a fairly clean, detectable signal, with CA-1 showing up along the brush border in mouse colon tissue. There were some cells that stained more intracellularly, and unsure if that was non-specific binding of the antibody, or another cell type other than colonocytes that expresses CA-1 intracellularly.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (WB) and 1:100 (IF)
  • Cell Tissue Type: Colon tissue (scrapes for western blot)
CA1 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB) and 1:100 (IF) dilution) in Colon tissue (scrapes for western blot) (Cat no:13198-2-AP)