• Featured Product
  • KD/KO Validated

CA12 Polyclonal antibody

CA12 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15180-1-AP


CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3

Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15180-1-AP targets CA12 in WB,IHC,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA12 fusion protein Ag7107
Full Name carbonic anhydrase XII
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 44 kDa, 75-80 kDa
GenBank Accession NumberBC000278
Gene Symbol CA12
Gene ID (NCBI) 771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.


Product Specific Protocols
WB protocol for CA12 antibody 15180-1-APDownload protocol
IHC protocol for CA12 antibody 15180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanWB, IF

J Exp Med

Large pH oscillations promote host defense against human airways infection.

Authors - Dusik Kim

Mol Oncol

Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling.

Authors - Maria B Asparuhova

Hum Mol Genet

Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease.

Authors - Melissa Lee

Int J Mol Sci

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.

Authors - Zihe Huo

J Enzyme Inhib Med Chem

Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.

Authors - Soyoung Hwang

Front Oncol

Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.

Authors - FeiYan Ai