CA12 Polyclonal antibody

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CA12 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15180-1-AP Copied!

Publications (22)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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  WB analysis using 15180-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected A549 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A549 using 15180-1-AP

  IP result of anti-CA12 (IP:15180-1-AP, 4ug; Detection:15180-1-AP 1:10000) with A549 cells lysate 840 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brown disease using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15180-1-AP (CA12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human brown disease, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15180-1-AP targets CA12 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7107

  Product name: Recombinant human CA12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-293 aa of BC000278

  Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI

• Full Name: carbonic anhydrase XII
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 44 kDa, 75-80 kDa
• GenBank Accession Number: BC000278
• Gene Symbol: CA12
• Gene ID (NCBI): 771
• RRID: AB_2065826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43570
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Publications

What published studies show

The CA12 Polyclonal antibody (Cat# 15180-1-AP) has 22 citations across 19 journals including Cell Stem Cell, Advanced Science, Journal of Experimental Medicine, Journal of Physiology, Molecular Oncology, Human Molecular Genetics, Frontiers in Oncology, and others. Research fields span cancer biology (breast, pancreatic, lung, colon, neuroblastoma, multiple myeloma), intervertebral disc degeneration, bicarbonate transport and ion channel biology, cardiotoxicity, wound repair, and hypoxia responses.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Stem Cell; CRISPRi/a screens in human iPSC-cardiomyocytes identify glycolytic activation as a druggable target for doxorubicin-induced cardiotoxicity; Authors - Chun Liu
human	WB	J Nanobiotechnology; Degenerated nucleus pulposus cells derived exosome carrying miR-27a-3p aggravates intervertebral disc degeneration by inducing M1 polarization of macrophages; Authors - Xin Zhao
human	WB	Adv Sci (Weinh); Estrogen-Induced LncRNA, LINC02568, Promotes Estrogen Receptor-Positive Breast Cancer Development and Drug Resistance Through Both In Trans and In Cis Mechanisms; Authors - Xue Chen
rat	IF	Adv Sci (Weinh); Preserving the Immune-Privileged Niche of the Nucleus Pulposus: Safeguarding Intervertebral Discs from Degeneration after Discectomy with Synthetic Mucin Hydrogel Injection; Authors - Huan Wang
human	WB,IF	J Exp Med; Large pH oscillations promote host defense against human airways infection.; Authors - Dusik Kim
human	WB	J Ethnopharmacol; Matrine injection inhibits pancreatic cancer growth via modulating carbonic anhydrases- a network pharmacology-based study with in vitro validation.; Authors - Pan-Ling Xu