CA12 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01993-1

  Sandwich ELISA standard curve of MP01993-1, Human CA12 Recombinant Matched Antibody Pair - PBS only. 85595-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7107. 85595-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Sample test of MP01993-1

  The mean CA12 concentration was determined to be 93.99 ng/mL in A549 cell extract based on a 3.10 mg/mL extract load.

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  Cytometric bead array standard curve of MP01993-1

  Cytometric bead array standard curve of MP01993-1, CA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85595-3-PBS. Detection antibody: 85595-2-PBS. Standard: Ag7107. Range: 0.781-100 ng/mL.

CA12 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85595-2-PBS

Clone No.243053A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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Product Information

85595-2-PBS targets CA12 as part of a matched antibody pair:

MP01993-1: 85595-3-PBS capture and 85595-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7107

  Product name: Recombinant human CA12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-293 aa of BC000278

  Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI

• Full Name: carbonic anhydrase XII
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: BC000278
• Gene Symbol: CA12
• Gene ID (NCBI): 771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43570
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.