CA2 Recombinant monoclonal antibody, PBS Only (Detector)

• 

  Sandwich ELISA standard curve of MP01702-2

  Sandwich ELISA standard curve of MP01702-2, Human CA2 Recombinant Matched Antibody Pair - PBS only. 84954-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10680. 84954-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

• 

  Sample test of MP01702-2

  The mean CA2 concentration was determined to be 872.48 ng/mL in HEK-293 cell extract based on a 2.70 mg/mL extract load, 444.36 ng/mL in U2OS cell extract based on a 3.10 mg/mL extract load and 499.13 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP01702-2

  Cytometric bead array standard curve of MP01702-2, CA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84954-1-PBS. Detection antibody: 84954-2-PBS. Standard: Ag10680. Range: 0.156-20 ng/mL.

CA2 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84954-2-PBS

Clone No.242559E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Car2, CA-II, CAII, CAC, CA II

---

Product Information

84954-2-PBS targets CA2 as part of a matched antibody pair:

MP01702-2: 84954-1-PBS capture and 84954-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10680

  Product name: Recombinant human CA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-260 aa of BC011949

  Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

• Full Name: carbonic anhydrase II
• Calculated Molecular Weight: 260 aa, 29 kDa
• GenBank Accession Number: BC011949
• Gene Symbol: CA2
• Gene ID (NCBI): 760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00918
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.