CA2 Recombinant antibody

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  WB analysis using 84954-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84954-4-RR (CA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84954-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84954-4-RR against Human CA2 were performed. The affinity constant is 23.5 nM.

CA2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84954-4-RR

Clone No.242559B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Car2, CA-II, CAII, CAC, CA II

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84954-4-RR targets CA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CA2 fusion protein Ag10680
• Full Name: carbonic anhydrase II
• Calculated Molecular Weight: 260 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC011949
• Gene Symbol: CA2
• Gene ID (NCBI): 760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00918
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).