CA3 Monoclonal antibody

CA3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat , Pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3C10A2

Cat no : 66608-1-Ig

Synonyms

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III



Tested Applications

Positive WB detected inpig skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66608-1-Ig targets CA3 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse , Rat , Pig samples.

Tested Reactivity Human, Mouse , Rat , Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CA3 fusion protein Ag7513
Full Name carbonic anhydrase III, muscle specific
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC004897
Gene symbol CA3
Gene ID (NCBI) 761
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

Protocols

Product Specific Protocols
WB protocol for CA3 antibody 66608-1-IgDownload protocol
IHC protocol for CA3 antibody 66608-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols