Tested Applications
| Positive WB detected in | mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13931-1-AP targets Carbonic Anhydrase 4/CA4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4960 Product name: Recombinant human CA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-284 aa of BC057792 Sequence: ASAESHWCYEVQAESSNYPCLVPVKWGGNCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISGGGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPEDEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYFRYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQLGQRTVIKS Predict reactive species |
| Full Name | carbonic anhydrase IV |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC057792 |
| Gene Symbol | CA4 |
| Gene ID (NCBI) | 762 |
| RRID | AB_2877990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22748 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressing on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Carbonic Anhydrase 4/CA4 antibody 13931-1-AP | Download protocol |
| WB protocol for Carbonic Anhydrase 4/CA4 antibody 13931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Carbonic Anhydrase 4/CA4 Polyclonal antibody (Cat# 13931-1-AP) has 5 citations published in Nature Communications, Theranostics, American Journal of Respiratory Cell and Molecular Biology, Scientific Reports, and Journal of Cancer. Research fields span mesenchymal stem cell biology, breast cancer subtype specification, lung injury and neutrophil activation, lizard chemical signaling, and colon adenocarcinoma prognosis. Applications cited include IHC, IF, and WB in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun A CCL2+DPP4+ subset of mesenchymal stem cells expedites aberrant formation of creeping fat in humans | ||
Theranostics Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype | ||
Am J Respir Cell Mol Biol Activated Clec4nhi Neutrophils Aggravate Lung Injury in an Endothelial IGFBP7 Dependent Manner | ||
J Cancer Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network. |
