CA4 Polyclonal antibody
CA4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13931-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse lung tissue, rat lung tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 2 publications below |
Product Information
13931-1-AP targets CA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CA4 fusion protein Ag4960 |
Full Name | carbonic anhydrase IV |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC057792 |
Gene Symbol | CA4 |
Gene ID (NCBI) | 762 |
RRID | AB_2877990 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressing on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.
Protocols
Product Specific Protocols | |
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WB protocol for CA4 antibody 13931-1-AP | Download protocol |
IHC protocol for CA4 antibody 13931-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype | ||
J Cancer Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network. | ||
Nat Commun A CCL2+DPP4+ subset of mesenchymal stem cells expedites aberrant formation of creeping fat in humans |