CA4 Polyclonal antibody

CA4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13931-1-AP

Synonyms

CA IV, CA4, CAIV, Car4, Carbonate dehydratase IV, Carbonic anhydrase 4, carbonic anhydrase IV, RP17



Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13931-1-AP targets CA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA4 fusion protein Ag4960
Full Name carbonic anhydrase IV
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC057792
Gene Symbol CA4
Gene ID (NCBI) 762
RRIDAB_2877990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressing on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.

Protocols

Product Specific Protocols
WB protocol for CA4 antibody 13931-1-APDownload protocol
IHC protocol for CA4 antibody 13931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Theranostics

Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype

Authors - Zhi-Jie Gao
WB

Sci Rep

Carbonic anhydrase IV in lizard chemical signals

Authors - Marco Mangiacotti
humanWB

J Cancer

Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network.

Authors - Wanpeng Wang
humanIHC

Nat Commun

A CCL2+DPP4+ subset of mesenchymal stem cells expedites aberrant formation of creeping fat in humans

Authors - Fengfei Wu