CA9 Monoclonal antibody

CA9 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G4G5

Cat no : 66243-1-Ig

Synonyms

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250



Tested Applications

Positive WB detected inhuman stomach tissue, pig stomach tissue, pig brain tissue, U-87 MG cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66243-1-Ig targets CA9 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CA9 fusion protein Ag1540
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014950
Gene symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549.

Protocols

Product Specific Protocols
WB protocol for CA9 antibody 66243-1-IgDownload protocol
IHC protocol for CA9 antibody 66243-1-IgDownload protocol
IF protocol for CA9 antibody 66243-1-IgDownload protocol
FC protocol for CA9 antibody 66243-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Oncol

Identification and validation of a combined hypoxia and immune index for triple-negative breast cancer.

Authors - Shaoquan Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (04-16-2019)

Specific band in hypoxic mouse kidney.Note: The lower part of the blot (<25 kDa) was stained for a hypoxic marker (Ndufa4l2).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney, hypoxic (lane1), normoxic (lane2)
CA9 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, hypoxic (lane1), normoxic (lane2) (Cat no:66243-1-Ig)