|Positive WB detected in||human stomach tissue, pig stomach tissue, pig brain tissue, U-87 MG cells|
|Positive IHC detected in||human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66243-1-Ig targets CA9 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.
|Tested Reactivity||human, mouse, pig, rabbit|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CA9 fusion protein Ag1540|
|Full Name||carbonic anhydrase IX|
|Calculated molecular weight||459 aa, 50 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC014950|
|Gene ID (NCBI)||768|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549.
|Product Specific Protocols|
|WB protocol for CA9 antibody 66243-1-Ig||Download protocol|
|IHC protocol for CA9 antibody 66243-1-Ig||Download protocol|
|IF protocol for CA9 antibody 66243-1-Ig||Download protocol|
|FC protocol for CA9 antibody 66243-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (04-16-2019)
Specific band in hypoxic mouse kidney.Note: The lower part of the blot (<25 kDa) was stained for a hypoxic marker (Ndufa4l2).