CAAP1 Polyclonal antibody

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• CAAP1 Polyclonal antibody (26213-1-AP)

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  WB analysis using 26213-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26213-1-AP (C9orf82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 26213-1-AP

  IP result of anti-C9orf82 (IP:26213-1-AP, 4ug; Detection:26213-1-AP 1:3000) with A431 cells lysate 800 ug.

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  IHC staining of human liver using 26213-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26213-1-AP (CAAP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26213-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26213-1-AP (CAAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CAAP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26213-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

C9orf82, CAAP, Caspase activity and apoptosis inhibitor 1, Conserved anti-apoptotic protein, RP11 337A23.1

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, MDA-MB-231 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26213-1-AP targets CAAP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23850

  Product name: Recombinant human C9orf82 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-361 aa of BC014658

  Sequence: MTGKKSSREKRRKRSSQEAAAALAAPDIVPALASGSSGSTSGCGSAGGCGSVSCCGNANFSGSVTGGGSGGSCWGGSSVERSERRKRRSTDSSSVSGSLQQETKYILPTLEKELFLAEHSDLEEGGLDLTVSLKPVSFYISDKKEMLQQCFCIIGEKKLQKMLPDVLKNCSIEEIKKLCQEQLELLSKKKILKILEGDNGMDSDMEEEADDGSKMGSDLVSQQDICIDSASSVRENKQPEGLELKQGKGEDSDVLSINADAYDSDIEGPCNEEAAAPEAPENTVQSEAGQIDDLEKDIEKSVNEILGLAESSPNEPKAATLAVPPPEDVQPSAQQLELLELEMRARAIKALMKAGDIKKPA

• Full Name: chromosome 9 open reading frame 82
• Observed Molecular Weight: 47-55 kDa
• GenBank Accession Number: BC014658
• Gene Symbol: C9orf82
• Gene ID (NCBI): 79886
• RRID: AB_3742173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H8G2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAAP1 (Caspase Activity and Apoptosis Inhibitor 1), also known as C9orf82, is an evolutionarily conserved anti-apoptotic protein. It inhibits apoptosis by interfering with the activation of caspase-10, thereby blocking the feedback amplification loop of caspase-3/9 in the mitochondrial apoptotic pathway. Recent studies have revealed that CAAP1 plays a dual role in the regulation of autophagy, balancing the processes of autophagy and apoptosis. In tumor biology, the expression level of CAAP1 is associated with chemotherapy resistance in various cancers, making it a potential prognostic biomarker. In addition, CAAP1 is involved in neuroprotective mechanisms and plays an important role in hypoxic-ischemic brain injury by regulating neuronal apoptosis. Its expression is finely regulated by non-coding RNA networks, including MKL1/miR-5100 and miR-184-5p. (PMID: 30629682)