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CABYR Polyclonal antibody

CABYR Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12351-1-AP

Synonyms

CABYR, Calcium binding protein 86, Cancer/testis antigen 88, CBP86, CT88, Fibrousheathin 2, Fibrousheathin II, FSP 2, FSP2



Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12351-1-AP targets CABYR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CABYR fusion protein Ag3012
Full Name calcium binding tyrosine-(Y)-phosphorylation regulated
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa, 86-90 kDa
GenBank accession numberBC011996
Gene symbol CABYR
Gene ID (NCBI) 26256
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CABYR antibody 12351-1-APDownload protocol
IHC protocol for CABYR antibody 12351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Clin Genet

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Authors - Yan-Wei Sha
humanWB

Nat Commun

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.

Authors - Ying Shen
mouseIF

J Cell Sci

Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice.

Authors - Ferheen Abbasi
MOUSEWB

J Cell Sci

CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.

Authors - Samantha A M Young
  • KO Validated