CABYR Polyclonal antibody

• Featured Product
• KD/KO Validated

CABYR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12351-1-AP

Synonyms

CABYR, Calcium binding protein 86, Cancer/testis antigen 88, CBP86, CT88, Fibrousheathin 2, Fibrousheathin II, FSP 2, FSP2

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Validation Data Gallery

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  WB analysis of human testis using 12351-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12351-1-AP (CABYR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12351-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12351-1-AP (CABYR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12351-1-AP (CABYR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12351-1-AP (CABYR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12351-1-AP (CABYR antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human pancreas cancer using 12351-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12351-1-AP (CABYR antibody) at dilution of 1:50 (under 25x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Product Information

12351-1-AP targets CABYR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CABYR fusion protein Ag3012
• Full Name: calcium binding tyrosine-(Y)-phosphorylation regulated
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa, 86-90 kDa
• GenBank Accession Number: BC011996
• Gene Symbol: CABYR
• Gene ID (NCBI): 26256
• RRID: AB_2069257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	J Genet Genomics; Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice; Authors - Yaling Wang
MOUSE	WB	J Cell Sci; CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.; Authors - Samantha A M Young ; KO Validated
mouse	IF	J Cell Sci; Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice.; Authors - Ferheen Abbasi
human,mouse	IF	Clin Genet; Biallelic mutations in PMFBP1 cause acephalic spermatozoa.; Authors - Yan-Wei Sha
human	WB	Nat Commun; Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.; Authors - Ying Shen
	WB	Animals (Basel); Comparative Study on the Sperm Proteomes of Horses and Donkeys; Authors - Hong Ren