CACNA1D Polyclonal antibody

CACNA1D Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22276-1-AP

Synonyms

CACH3, CACN4, CACNA1D, CACNL1A2, Cav1.3, CCHL1A2



Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

22276-1-AP targets CACNA1D in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium channel, voltage-dependent, L type, alpha 1D subunit
Calculated molecular weight 248 kDa
Observed molecular weight 200-250 kDa
GenBank accession numberNM_000720
Gene symbol CACNA1D
Gene ID (NCBI) 776
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CACNA1D antibody 22276-1-APDownload protocol
IHC protocol for CACNA1D antibody 22276-1-APDownload protocol
IF protocol for CACNA1D antibody 22276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols