Filter: AllIHCIF-PIF

IHC staining of human cerebellum using 17821-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17821-1-AP (CACNA1G Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cerebellum using 17821-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17821-1-AP (CACNA1G Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 17821-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17821-1-AP (CACNA1G Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 17821-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17821-1-AP (CACNA1G Antibody) at dilution of 1:100 (under 40x lens).
IF Staining of mouse brain using 17821-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CACNA1G antibody (17821-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat brain using 17821-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CACNA1G antibody (17821-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 17821-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CACNA1G antibody (17821-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart tissue using 17821-1-AP
IF result of anti-CACNA1G (17821-1-AP) in frozen mouse heart section by Dr Nicola Smart.

CACNA1G Polyclonal antibody

CACNA1G Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 17821-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

Ca(V)T.1, Cav3.1, Cav3.1c, KIAA1123, NBR13

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human cerebellum tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue, mouse brain tissue, rat brain tissue

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below
IF	See 2 publications below

Product Information

17821-1-AP targets CACNA1G in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12188 Product name: Recombinant human CACNA1G protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 396-742 aa of BC110995 Sequence: TQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPLGGQETQPSSSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDRDANGSRRLMLPPPSTPALSGAPPGGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPETLKEKALVEVAASSGPPTLTSLNIPPGPYSSMHKLLETQSTGACQSSCKISSPCLKADSGACGPDSCPYCARAGAGEVELADREMPDSDSEAVYEFTQDAQHSDLRDPHSRRQRSLGPDAEPSSVLAFWRLICDTFRKIVDS Predict reactive species
Full Name	calcium channel, voltage-dependent, T type, alpha 1G subunit
Calculated Molecular Weight	2261aa,250 kDa; 2377aa,263 kDa
GenBank Accession Number	BC110995
Gene Symbol	CACNA1G
Gene ID (NCBI)	8913
RRID	AB_2878446
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43497
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CACNA1G antibody 17821-1-AP	Download protocol
IHC protocol for CACNA1G antibody 17821-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	J Perinat Med Cardiac ion channels associated with unexplained stillbirth - An immunohistochemical study. Authors - Susana Quesado Branco View Article
mouse	IF	Oxid Med Cell Longev NRF-2/HO-1 Pathway-Mediated SHOX2 Activation Is a Key Switch for Heart Rate Acceleration by Yixin-Fumai Granules Authors - Heng Zhang View Article
mouse	IF	Aging (Albany NY) D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism Authors - Heng Zhang View Article
Mouse		iScience IGF2BP2-Shox2 axis regulates hippocampal-neuronal senescence to alleviate microgravity-induced recognition disturbance Authors - Yujie Zhao View Article