CACNA1S Polyclonal antibody

CACNA1S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22279-1-AP

Synonyms

CACH1, CACN1, CACNA1S, CACNL1A3, Cav1.1, CCHL1A3, HOKPP, hypoPP, MHS5

---

Validation Data Gallery

• 

  WB analysis of human brain using 22279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22279-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22279-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

22279-1-AP targets CACNA1S in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
• Calculated Molecular Weight: 212 kDa
• Observed Molecular Weight: 200-220 kDa
• GenBank Accession Number: NM_000069
• Gene Symbol: CACNA1S
• Gene ID (NCBI): 779
• RRID: AB_2879058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACNA1S, also named as CACH1, CACN1 and CACNL1A3, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family and CACNA1S subfamily. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. The antibody is specific to CACNA1S.

Publications

Species	Application	Title
human	IHC	Reproduction; Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.; Authors - Ru Zheng
human	WB	bioRxiv; Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM; Authors - Jorge L Jimenez-Macias