CACYBP Monoclonal antibody

CACYBP Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C2G11

Cat no : 68161-1-Ig

Synonyms

CACYBP, calcyclin binding protein, GIG5, hCacyBP, PNAS 107, RP1 102G20.6, S100A6 binding protein, S100A6BP, Siah interacting protein, SIP



Tested Applications

Positive WB detected inHCT 116 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

68161-1-Ig targets CACYBP in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CACYBP fusion protein Ag2340
Full Name calcyclin binding protein
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight26-30 kDa
GenBank accession numberBC022352
Gene symbol CACYBP
Gene ID (NCBI) 27101
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

Protocols

Product Specific Protocols
WB protocol for CACYBP antibody 68161-1-IgDownload protocol
IF protocol for CACYBP antibody 68161-1-IgDownload protocol
IP protocol for CACYBP antibody 68161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols