• Featured Product
  • KD/KO Validated

CALCOCO1 Polyclonal antibody

CALCOCO1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19843-1-AP

Synonyms

CALCOCO1, calphoglin, Cocoa, KIAA1536, PP13275, Sarcoma antigen NY SAR 3, UNQ2436/PRO4996



Tested Applications

Positive WB detected inHT-1080 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19843-1-AP is CALCOCO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCOCO1 fusion protein Ag13876
Full Name calcium binding and coiled-coil domain 1
Calculated molecular weight 691 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC003177
Gene symbol CALCOCO1
Gene ID (NCBI) 57658
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CALCOCO1 antibody 19843-1-APDownload protocol
IHC protocol for CALCOCO1 antibody 19843-1-APDownload protocol
IF protocol for CALCOCO1 antibody 19843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Signal

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Authors - Lisa M Cooper
mouseWB

Elife

Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.

Authors - Junghyun Lim
  • KO Validated