CALCOCO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CALCOCO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19843-1-AP

Synonyms

Sarcoma antigen NY-SAR-3, PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin

---

Validation Data Gallery

• 

  WB analysis using 19843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19843-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19843-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALCOCO1 antibody (19843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

19843-1-AP targets CALCOCO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALCOCO1 fusion protein Ag13876
• Full Name: calcium binding and coiled-coil domain 1
• Calculated Molecular Weight: 691 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC003177
• Gene Symbol: CALCOCO1
• Gene ID (NCBI): 57658
• RRID: AB_10637265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Elife; Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.; Authors - Junghyun Lim ; KO Validated
mouse	WB	Cell Signal; Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.; Authors - Lisa M Cooper
mouse	WB	Nat Commun; Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer; Authors - Lucia Taraborrelli