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  • KD/KO Validated

CALCOCO1 Polyclonal antibody

CALCOCO1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19843-1-AP

Synonyms

CALCOCO1, calphoglin, Cocoa, KIAA1536, PP13275, Sarcoma antigen NY SAR 3, UNQ2436/PRO4996



Tested Applications

Positive WB detected inHT-1080 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19843-1-AP is CALCOCO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCOCO1 fusion protein Ag13876
Full Name calcium binding and coiled-coil domain 1
Calculated molecular weight 691 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC003177
Gene symbol CALCOCO1
Gene ID (NCBI) 57658
RRIDAB_10637265
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CALCOCO1 antibody 19843-1-APDownload protocol
IHC protocol for CALCOCO1 antibody 19843-1-APDownload protocol
IF protocol for CALCOCO1 antibody 19843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.

Authors - Junghyun Lim
  • KO Validated
mouseWB

Cell Signal

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Authors - Lisa M Cooper
mouseWB

Nat Commun

Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer

Authors - Lucia Taraborrelli