CALCOCO1 Polyclonal antibody

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  WB analysis using 19843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19843-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALCOCO1 antibody (19843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

CALCOCO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19843-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Calcium-binding and coiled-coil domain-containing protein 1, Calphoglin, Cocoa, Coiled-coil coactivator protein, PP13275

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Tested Applications

• Positive WB detected in: HEK-293T cells, HT-1080 cells, HeLa cells, MCF-7 cells, Ramos cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue, human kidney tissue, mouse brain tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

19843-1-AP targets CALCOCO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13876

  Product name: Recombinant human CALCOCO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 341-691 aa of BC003177

  Sequence: LKEQLRGAQELAASSQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVEAEKDKILKLSAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLPPYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAEDEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPATPTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFE

• Full Name: calcium binding and coiled-coil domain 1
• Calculated Molecular Weight: 691 aa, 77 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC003177
• Gene Symbol: CALCOCO1
• Gene ID (NCBI): 57658
• RRID: AB_10637265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P1Z2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Elife; Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.; Authors - Junghyun Lim ; KO Validated
mouse	WB	Cell Signal; Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.; Authors - Lisa M Cooper
mouse	WB	Nat Commun; Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer; Authors - Lucia Taraborrelli