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  • KD/KO Validated

NDP52 Polyclonal antibody

NDP52 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12229-1-AP

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52



Tested Applications

Positive WB detected inHeLa cells, rat skeletal muscle tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12229-1-AP targets NDP52 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDP52 fusion protein Ag2866
Full Name calcium binding and coiled-coil domain 2
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_11182600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
WB protocol for NDP52 antibody 12229-1-APDownload protocol
IHC protocol for NDP52 antibody 12229-1-APDownload protocol
IF protocol for NDP52 antibody 12229-1-APDownload protocol
IP protocol for NDP52 antibody 12229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

Authors - Zhenyu Zhong
humanWB

Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KO Validated
humanWB

Autophagy

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression

Authors - Tai-Mei Zhang
humanWB

Nat Commun

TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.

Authors - Minwoo Baek
mouseWB

Nat Commun

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Authors - Insup Choi
mouseWB

Redox Biol

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease

Authors - Yi Sun