CALCR Polyclonal antibody

CALCR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20868-1-AP

Synonyms

CTR1, CT-R, CTR, CT R, CRT

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 20868-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20868-1-AP (CALCR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20868-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20868-1-AP (CALCR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: MCF-7 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

20868-1-AP targets CALCR in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALCR fusion protein Ag14920
• Full Name: calcitonin receptor
• Calculated Molecular Weight: 490 aa, 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC075028
• Gene Symbol: CALCR
• Gene ID (NCBI): 799
• RRID: AB_2878754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CALCR protein, also known as the calcitonin receptor, is a high-affinity receptor for the peptide hormone calcitonin and belongs to the subfamily of seven transmembrane-spanning G protein-coupled receptors. This protein plays a critical role in maintaining calcium homeostasis and regulating osteoclast-mediated bone resorption. The calcitonin receptor is involved in several physiological processes, including inhibition of osteoclastic bone resorption and increased renal calcium excretion, which helps to regulate Ca2+ concentrations. In addition, the CALCR protein has been detected in a variety of tissues not directly involved in the regulation of calcium homeostasis, with particular expression observed in the brain, suggesting distinct functions in the central nervous system (CNS) (PMID: 12730726).

Publications

Species	Application	Title
	WB	J Cell Mol Med; Cytisine attenuates bone loss of ovariectomy mouse by preventing RANKL-induced osteoclastogenesis.; Authors - Zhi Qian
human	WB	Mol Med; A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone.; Authors - Yu Fu
human	WB	Curr Issues Mol Biol; COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer; Authors - Ming-Wei Lin
human	WB	Adv Sci (Weinh); Enhanced Protein Damage Clearance Induces Broad Drug Resistance in Multitype of Cancers Revealed by an Evolution Drug-Resistant Model and Genome-Wide siRNA Screening.; Authors - Fangyuan Shao
mouse	WB	Nat Commun; Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics; Authors - Xi Shao
mouse	WB	Cell Metab; Gut microbial alterations in arginine metabolism determine bone mechanical adaptation; Authors - Dan Wang