CALN1 Polyclonal antibody

CALN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11477-1-AP

Synonyms

CaBP8, Calcium binding protein 8, CALN1, calneuron 1, Calneuron I

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Validation Data Gallery

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  WB analysis of rat brain using 11477-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11477-1-AP (CALN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11477-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11477-1-AP (CALN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11477-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11477-1-AP (CALN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 11477-1-AP

  IP result of anti-CALN1 (IP:11477-1-AP, 4ug; Detection:11477-1-AP 1:300) with rat brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 11477-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11477-1-AP (CALN1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: rat brain tissue, human brain tissue, mouse brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11477-1-AP targets CALN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALN1 fusion protein Ag2043
• Full Name: calneuron 1
• Calculated Molecular Weight: 219 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC020200
• Gene Symbol: CALN1
• Gene ID (NCBI): 83698
• RRID: AB_2228454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; circEYA1 Functions as a Sponge of miR-582-3p to Suppress Cervical Adenocarcinoma Tumorigenesis via Upregulating CXCL14.; Authors - Junfen Xu
rat	WB, IHC	Hypertension; Calneuron 1 Increased Ca2+ in the Endoplasmic Reticulum and Aldosterone Production in Aldosterone-Producing Adenoma.; Authors - Kazuhiro Kobuke
monkey	WB,IF	J Biol Chem; Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone.; Authors - Hradsky Johannes J
mouse	WB	J Cell Mol Med; Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network; Authors - Zhi-Hang Huang
human	WB,IHC	Biochem Biophys Res Commun; Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1.; Authors - Liangzhi Gong