CALN1 Polyclonal antibody

CALN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11477-1-AP

Synonyms

CaBP8, Calcium binding protein 8, CALN1, calneuron 1, Calneuron I



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, mouse brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11477-1-AP targets CALN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALN1 fusion protein Ag2043
Full Name calneuron 1
Calculated Molecular Weight 219 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC020200
Gene Symbol CALN1
Gene ID (NCBI) 83698
RRIDAB_2228454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CALN1 antibody 11477-1-APDownload protocol
IHC protocol for CALN1 antibody 11477-1-APDownload protocol
IP protocol for CALN1 antibody 11477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

circEYA1 Functions as a Sponge of miR-582-3p to Suppress Cervical Adenocarcinoma Tumorigenesis via Upregulating CXCL14.

Authors - Junfen Xu
ratWB, IHC

Hypertension

Calneuron 1 Increased Ca2+ in the Endoplasmic Reticulum and Aldosterone Production in Aldosterone-Producing Adenoma.

Authors - Kazuhiro Kobuke
monkeyWB,IF

J Biol Chem

Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone.

Authors - Hradsky Johannes J
mouseWB

J Cell Mol Med

Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network

Authors - Zhi-Hang Huang
humanWB,IHC

Biochem Biophys Res Commun

Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1.

Authors - Liangzhi Gong