Calumenin Polyclonal antibody

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  WB analysis of HEK-293 using 17804-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17804-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17804-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17804-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (17804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Calumenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17804-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CALU, Calumenin, Crocalbin, IEF SSP 9302

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, human placenta tissue, K-562 cells, mouse kidney tissue
• Positive IHC detected in: human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

17804-1-AP targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12073

  Product name: Recombinant human CALU protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-315 aa of BC013383

  Sequence: MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF

• Full Name: calumenin
• Calculated Molecular Weight: 315 aa, 37 kDa
• Observed Molecular Weight: 47 kDa, 57 kDa
• GenBank Accession Number: BC013383
• Gene Symbol: Calumenin
• Gene ID (NCBI): 813
• RRID: AB_2069964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43852
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Oncogene; Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis.; Authors - Q Wang ; KD Validated
human	IF	Cancer Med; Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer.; Authors - Mili Zhang
pig	WB	Cell Tissue Res; Hormone-regulated PKA activity in porcine oviductal epithelial cells.; Authors - Juan Manuel Teijeiro
human	WB,IHC,IF	J Inflamm Res; Pan-Cancer Screening and Validation of CALU's Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer; Authors - Shi-Liang Chen
human	WB	Respir Res; CALU promotes lung adenocarcinoma progression by enhancing cell proliferation, migration and invasion; Authors - Yan Li