CAMK1 Polyclonal antibody

CAMK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24312-1-AP

Synonyms

CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha

---

Validation Data Gallery

• 

  WB analysis using 24312-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24312-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of HEK-293 using 24312-1-AP

  IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:300) with HEK-293 cells lysate 3400ug.

• 

  IP experiment of HEK-293 using 24312-1-AP

  IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:500) with HEK-293 cells lysate 2960 ug.

• 

  IHC staining of human colon cancer using 24312-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human colon cancer using 24312-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human breast cancer using 24312-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24312-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 24312-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 24312-1-AP (CAMK1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 24312-1-AP is CAMK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMK1 fusion protein Ag19449
• Full Name: calcium/calmodulin-dependent protein kinase I
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC106755
• Gene Symbol: CAMK1
• Gene ID (NCBI): 8536
• RRID: AB_2879489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biomed Res Int; Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells.; Authors - Huilei Zheng
mouse	IHC	Biochim Biophys Acta Mol Basis Dis; IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy; Authors - Du Yuan