CAMK1D Polyclonal antibody

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• CAMK1D Polyclonal antibody (13613-1-AP)

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• KD/KO Validated

CAMK1D Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13613-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase type 1D, CaM K1, CaM KI delta, CaM kinase I delta, CaM kinase ID

• 

  WB analysis of HeLa using 13613-1-AP

  WB result of CAMK1D antibody (13613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMK1D transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 13613-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 13613-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, HeLa cells, mouse thymus tissue, rat pancreas tissue
• Positive IHC detected in: mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13613-1-AP targets CAMK1D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4517

  Product name: Recombinant human CAMK1D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4-198 aa of BC035745

  Sequence: ENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPY

• Full Name: calcium/calmodulin-dependent protein kinase ID
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC035745
• Gene Symbol: CAMK1D
• Gene ID (NCBI): 57118
• RRID: AB_2228498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IU85
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMK1D (calcium/calmodulin-dependent protein kinase ID) is a serine/threonine kinase belonging to the calcium/calmodulin-dependent protein kinase 1 family. It functions as a component of a calcium-triggered signaling cascade, playing roles in regulating granulocyte function, activating CREB-dependent gene transcription, and influencing neutrophil chemotaxis and apoptosis. CAMK1D is involved in diverse biological processes including nervous system development and inflammatory responses.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Podocyte-specific KLF6 primes proximal tubule CaMK1D signaling to attenuate diabetic kidney disease; Authors - Nehaben A Gujarati