Filter: AllWBIHCIF/ICC

WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of pig cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of rat cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of mouse cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66843-1-Ig (same clone as 66843-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66843-1-Ig (same clone as 66843-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

CaMKII alpha Monoclonal antibody, PBS Only

CaMKII alpha Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66843-1-PBS

Clone No.1E3B3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMK-II subunit alpha

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

66843-1-PBS targets CaMKII alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag4736 Product name: Recombinant human CAMK2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-271 aa of BC040457 Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI Predict reactive species
Full Name	calcium/calmodulin-dependent protein kinase II alpha
Calculated Molecular Weight	478 aa, 54 kDa
Observed Molecular Weight	45-60 kDa
GenBank Accession Number	BC040457
Gene Symbol	CaMKII alpha
Gene ID (NCBI)	815
RRID	AB_2882184
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9UQM7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa. This antibody can recognize all the members of the CAMK2 family.