Filter: AllWBIHCIF/ICC

WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of pig cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of rat cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of mouse cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66843-1-Ig (same clone as 66843-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66843-1-Ig (same clone as 66843-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

CAMK2 Monoclonal antibody, PBS Only

CAMK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66843-1-PBS

Clone No.1E3B3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMKA, CaMKII alpha, CaMK-II subunit alpha

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66843-1-PBS targets CAMK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CAMK2 fusion protein Ag4736 Predict reactive species
Full Name	calcium/calmodulin-dependent protein kinase II alpha
Calculated Molecular Weight	478 aa, 54 kDa
Observed Molecular Weight	45-60 kDa
GenBank Accession Number	BC040457
Gene Symbol	CaMKII alpha
Gene ID (NCBI)	815
RRID	AB_2882184
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UQM7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.