Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue, pig brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81014-1-RR targets CaMKII delta in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag31276 Product name: Recombinant human CAMK2D protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 249-445 aa of BC032784 Sequence: INKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPK Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase II delta |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 54-59 kDa |
| GenBank Accession Number | BC032784 |
| Gene Symbol | CAMK2D |
| Gene ID (NCBI) | 817 |
| RRID | AB_2923696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13557 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaMKII delta, also named as CAMKD, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It may also recognize CAMK2A due to the high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CaMKII delta antibody 81014-1-RR | Download protocol |
| WB protocol for CaMKII delta antibody 81014-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CaMKII delta Recombinant monoclonal antibody (1M10), catalog number 81014-1-RR, has 1 total citation. It appears in the Journal of Translational Medicine (J Transl Med, IF 9.7). The cited study investigates F-box protein FBXO21 in clear cell renal cell carcinoma, exploring its role in inhibiting proliferation and metastasis through the CREB pathway and tumor immune cell infiltration. The research field is oncology/renal cell carcinoma.
| Species | Application | Title |
|---|---|---|
J Transl Med F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration |
