Tested Applications
| Positive WB detected in | SMMC-7721 cells, human brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12666-2-AP targets CaMKII Gamma in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3348 Product name: Recombinant human CAMK2G protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 218-527 aa of BC034044 Sequence: DQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSVGRQSSAPASPAASAAGLAGQAAKSLLNKKSDGGVKKRKSSSSVHLMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAPAAPLQ Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase II gamma |
| Calculated Molecular Weight | 527 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC034044 |
| Gene Symbol | CAMK2G |
| Gene ID (NCBI) | 818 |
| RRID | AB_2068213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IHC protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IP protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| WB protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CaMKII Gamma Polyclonal antibody (catalog number 12666-2-AP) has 50 citations. These appear in journals including Signal Transduction and Targeted Therapy, Advanced Materials, Nature Communications, ACS Nano, Neuron, Theranostics, Cell Death & Disease, EMBO Journal, Free Radical Biology and Medicine, Oncotarget, Development, and the Journal of Biological Chemistry. Research fields span cancer biology, neuroscience, cardiovascular disease, inflammation, bone and cartilage regeneration, pain, and cellular signaling pathways.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. | ||
Adv Mater Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects | ||
Adv Mater Mechano-Iontronic Hydrogels Generating Biomimetic Endogenous Bioelectricity for Promoting Cartilage Regeneration. | ||
Nat Commun An attention-demanding hunting paradigm engages the superior colliculus-zona incerta circuit mediating analgesia in male mice. | ||
ACS Nano In Situ Reprogramming of Senescent Microenvironment via Augmentation of Endogenous Senolytic Actions for Advanced Atherosclerosis Management. | ||
Neuron JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse. |
