CaMKII Gamma Polyclonal antibody

• Featured Product
• KD/KO Validated

CaMKII Gamma Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 12666-2-AP

Synonyms

CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 12666-2-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 12666-2-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12666-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12666-2-AP

  IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug.

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  IHC staining of mouse heart using 12666-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12666-2-AP (CaMKII Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12666-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 12666-2-AP

  Immunofluorescent analysis of HepG2 cells, using CAMK2G antibody 12666-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, human brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12666-2-AP targets CaMKII Gamma in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CaMKII Gamma fusion protein Ag3348
• Full Name: calcium/calmodulin-dependent protein kinase II gamma
• Calculated Molecular Weight: 527 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC034044
• Gene Symbol: CAMK2G
• Gene ID (NCBI): 818
• RRID: AB_2068213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.

Publications

Species	Application	Title
mouse	WB	Neuron; JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.; Authors - Jamee M Berg
mouse	WB	Transl Res; Cardiac-Specific Overexpression of CREM-IbΔC-X via CRISPR/Cas9 in Mice Presents a New Model of Atrial Cardiomyopathy with Spontaneous Atrial Fibrillation; Authors - Jiuzhou Chen
human	WB	Oncotarget; PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.; Authors - Chao Li
mouse	IF	Development; Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse.; Authors - Chang Heng-Yu HY
human	WB,IF	Aging (Albany NY); CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis.; Authors - Linlin Yang ; KD Validated
human	WB	Cell Commun Signal; Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression.; Authors - Qing Liu