CAMKK1 Polyclonal antibody

CAMKK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13624-1-AP

Synonyms

CaM KK 1, CaM kinase kinase alpha, CaM kinase kinase 1, CaM kinase IV kinase, Calcium/calmodulin-dependent protein kinase kinase alpha

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Validation Data Gallery

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  WB analysis of Jurkat using 13624-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13624-1-AP

  IP result of anti-CAMKK1 (IP:13624-1-AP, 4ug; Detection:13624-1-AP 1:1000) with Jurkat cells lysate 4000ug.

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  IHC staining of human stomach using 13624-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13624-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMKK1 fusion protein Ag4534
• Full Name: calcium/calmodulin-dependent protein kinase kinase 1, alpha
• Calculated Molecular Weight: 520 aa, 57 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC031647
• Gene Symbol: CAMKK1
• Gene ID (NCBI): 84254
• RRID: AB_2068403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcium/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda and involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory.This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 .