CAMKV Polyclonal antibody

CAMKV Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 14788-1-AP

Synonyms

VACAMKL, CaM kinase-like vesicle-associated protein

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Validation Data Gallery

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  WB analysis of mouse brain using 14788-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14788-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14788-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14788-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14788-1-AP

  IP result of anti-CAMKV (IP:14788-1-AP, 4ug; Detection:14788-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of mouse brain using 14788-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14788-1-AP (CAMKV antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse spleen tissue, PC-3 cells
• Positive IP detected in: mouse brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14788-1-AP targets CAMKV in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMKV fusion protein Ag6438
• Full Name: CaM kinase-like vesicle-associated
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-75 kDa
• GenBank Accession Number: BC000497
• Gene Symbol: CAMKV
• Gene ID (NCBI): 79012
• RRID: AB_2068552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).

Publications

Species	Application	Title
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen
human,mouse	WB,IHC,IF	Ann Neurol; Paraneoplastic Calmodulin Kinase-Like Vesicle-Associated Protein (CAMKV) Autoimmune Encephalitis; Authors - Michael Gilligan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

María Inmaculada (Verified Customer) (07-15-2022)

I used the antibody for Western Blot and it worked very well. First, we used it at a 1:500 dilution first, but it was super concentrated and obtained unspecific bands. Then, I used it at a 1:2500 dilution and obtained pretty clean bands for the protein. The signal was very intense with my system, so I think I could have even diluted it a bit more.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Mouse brain