CAMSAP1 Recombinant antibody

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  WB analysis using 83864-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83864-1-RR (CAMSAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Neuro-2a using 83864-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CAMSAP1 antibody (83864-1-RR, Clone: 240633H1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CAMSAP1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83864-1-RR

Clone No.240633H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PRO2405, Calmodulin-regulated spectrin-associated protein 1, 240633H1

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Tested Applications

• Positive WB detected in: MCF-7 cells, Neuro-2a cells
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83864-1-RR targets CAMSAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: calmodulin regulated spectrin-associated protein 1
• Calculated Molecular Weight: 179 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC144082
• Gene Symbol: CAMSAP1
• Gene ID (NCBI): 157922
• RRID: AB_3671450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5T5Y3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are three members in the CAMSAP family of mammals, namely CAMSAP1-3. Despite having similar structures, these proteins have different distribution patterns within intracellular spaces and tissues. CAMSAP1 (Calmodulin-regulated spectrin-associated protein 1) is mainly expressed in nervous tissue, testis, and liver (PMID: 37903275).