CANT1 Polyclonal antibody

• 

  WB analysis using 12164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12164-1-AP (CANT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12164-1-AP (CANT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

CANT1 Polyclonal Antibody for WB, ELISA

Cat No. 12164-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Apyrase homolog, CANT1, SCAN 1, SHAPY

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Tested Applications

• Positive WB detected in: U2OS cells, A549 cells, mouse lung tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12164-1-AP targets CANT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2805

  Product name: Recombinant human CANT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 71-401 aa of BC017655

  Sequence: THNAHNWRLGQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI

• Full Name: calcium activated nucleotidase 1
• Calculated Molecular Weight: 401 aa, 45 kDa
• Observed Molecular Weight: 39-40 kDa
• GenBank Accession Number: BC017655
• Gene Symbol: CANT1
• Gene ID (NCBI): 124583
• RRID: AB_10639511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVQ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	BMC Complement Med Ther; Integrated proteomics and metabolomics analysis of lumbar in a rat model of osteoporosis treated with Gushukang capsules; Authors - Ruohui Lin