Tested Applications
| Positive WB detected in | Caco-2 cells, COLO 320 cells, HepG2 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16231-1-AP targets CAP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8876 Product name: Recombinant human CAP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 125-475 aa of BC013963 Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG Predict reactive species |
| Full Name | CAP, adenylate cyclase-associated protein 1 (yeast) |
| Calculated Molecular Weight | 475 aa, 52 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC013963 |
| Gene Symbol | CAP1 |
| Gene ID (NCBI) | 10487 |
| RRID | AB_2172166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01518 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAP1 antibody 16231-1-AP | Download protocol |
| IP protocol for CAP1 antibody 16231-1-AP | Download protocol |
| WB protocol for CAP1 antibody 16231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAP1 Polyclonal antibody (Cat# 16231-1-AP) has 6 citations across six journals: PNAS, J Med Chem, Cell Mol Life Sci, J Biol Chem, Viruses, and Cell Biochem Funct. Research fields span adenylyl cyclase activation, proteome-wide target discovery and protein degradation, actin regulation in dendritic spines, Rap1 GTPase binding, viral immunology (PCV2/SIGIRR), and bovine mammary epithelial cell biology. All citations used WB, with one also employing IF.
| Species | Application | Title |
|---|---|---|
J Med Chem Azoacetylene Electrophiles for Proteome-wide Ligand and Target Discovery: Supporting Targeted Protein Degradation | ||
Cell Mol Life Sci Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology | ||
J Biol Chem Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase.
| ||
Viruses Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage. | ||
Cell Biochem Funct Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.
|
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1,000 dilution and reported positive results. One user tested it in human coronary artery endothelial cells and described excellent performance, expressing satisfaction with the antibody's reliability. The other user tested it in hippocampal homogenate and observed a sharp band just over 50 kDa. Overall, the reviews reflect consistent, high-quality performance across different tissue types, with an average rating of 4.5 out of 5.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bruna (Verified Customer) (10-28-2026) | I recently tested the CAP1 and CAP2 antibodies in Human Coronary Artery Endothelial Cells (HCAEC), and both worked beautifully! I obtained excellent results using a 1:1,000 dilution. I'm very pleased with their performance and wanted to share my positive experience. Thank you for providing such reliable antibodies!
 |
Kuldeep (Verified Customer) (09-17-2025) | sharp band, little over 50KDa.
 |
