Calpain 2 Polyclonal antibody

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Calpain 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11472-1-AP Copied!

Publications (29)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CAPN2, Calpain M-type, Calpain M type, Calpain large polypeptide L2, Calpain 2 large subunit

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  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 (WT and CAPN2 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 11472-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 11472-1-AP

  WB result of Calpain 2 antibody (11472-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 2 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Y79 using 11472-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 11472-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 lysates prepared and IP of CAPN2 performed using 1.0 μg of 11472-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human kidney using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11472-1-AP (Calpain 2 antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11472-1-AP (Calpain 2 antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 11472-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Calpain 2 antibody (11472-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11472-1-AP at 1:300, plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Tested Applications

• Positive WB detected in: A549 cells, MDA-MB-231 cells, human placenta tissue, HeLa cells, Y79 cells, C2C12 cells, mouse brain tissue, rat brain tissue, K-562 cells, PC-3 cells
• Positive IP detected in: MDA-MB-231 cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11472-1-AP targets Calpain 2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2029

  Product name: Recombinant human CAPN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC021303

  Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD

• Full Name: calpain 2, (m/II) large subunit
• Calculated Molecular Weight: 700 aa, 80 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC021303
• Gene Symbol: CAPN2
• Gene ID (NCBI): 824
• RRID: AB_10755306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17655
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

Publications

What published studies show

The Calpain 2 Polyclonal antibody (Cat# 11472-1-AP) has 29 citations. They appear in journals including Advanced Science, Pharmacological Research, Cell Communication & Signaling, Phytomedicine, Journal of Translational Medicine, Oncogene, International Journal of Surgery, Molecular Medicine, Biomedical Pharmacotherapy, JCI Insight, Journal of the American Heart Association, International Immunopharmacology, Molecular Neurobiology, Frontiers in Pharmacology, Arthritis Research & Therapy, Biomedicines, Fish & Shellfish Immunology, Molecular Immunology, Toxicology & Applied Pharmacology, PeerJ, Annals of Anatomy, Experimental & Therapeutic Medicine, Physiological Reports, and European Journal of Pharmacology. Research fields span cancer, cardiovascular disease, neurology, fibrosis, and ferroptosis.

Automatically generated from the 29 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IP,IF	Adv Sci (Weinh); Calpain-2-Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection; Authors - Xiaomei Teng
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang
mouse	IF	Pharmacol Res; TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.; Authors - Wen Tian
human	IF	Cell Commun Signal; CAPNS1/Dicer feedback loop facilitates glycolysis, proliferation, and metastasis in hepatocellular carcinoma cells by inhibiting the maturation of MicroRNA pool.; Authors - Zhirui Zeng
rat	WB,IHC,IF	Phytomedicine; Bufei Jianspi formula alleviates intestinal epithelial barrier injury by inhibition of calpain 2 in COPD rat.; Authors - Kun Wang
human	WB,IHC	Phytomedicine; Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells; Authors - Maolei Xu

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

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Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Wei (Verified Customer) (03-12-2020)

Weak but clear band for western blot on mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart