Calpain 6 Polyclonal antibody

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  WB analysis using 10120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 10120-1-AP

  Mouse placenta tissue was subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10120-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 10120-1-AP

  IP result of anti-Calpain 6 (IP:10120-1-AP, 4ug; Detection:10120-1-AP 1:300) with human placenta tissue lysate 1520ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10120-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10120-1-AP (Calpain 6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calpain 6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10120-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CAPN6, Calpain-like protease X-linked, Calpain-6, calpain6

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse placenta tissue, A549 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10120-1-AP targets Calpain 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0160

  Product name: Recombinant human CAPN6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 67-360 aa of BC000730

  Sequence: NISNHQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQKTEKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEEKYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLGERLVEVFSAEKVYMVRLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDFCRNFHKLNVCRNVNNPIFGRKELESVLGCW

• Full Name: calpain 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 66-75 kDa
• GenBank Accession Number: BC000730
• Gene Symbol: Calpain 6
• Gene ID (NCBI): 827
• RRID: AB_2290829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6Q1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IF	J Neuropathol Exp Neurol; MicroRNA Alteration in Developing Rat Oligodendrocyte Precursor Cells Induced by Hypoxia-Ischemia.; Authors - Xiaojuan Su
human	WB,IHC	Am J Reprod Immunol; The investigation of calpain in human placenta with fetal growth restriction.; Authors - Shinobu Goto
human	WB	Amino Acids; Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6; Authors - Yufan Zhang
human	IF	Cell Biosci; Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos; Authors - Qianqi Zhu