Calpain 6 Polyclonal antibody

Calpain 6 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10120-1-AP

Synonyms

calpain 6, Calpain like protease X linked, Calpamodulin, CalpM, CANPX, CAPN6, CAPNX

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Validation Data Gallery

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  WB analysis using 10120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 10120-1-AP

  Mouse placenta tissue was subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10120-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 10120-1-AP

  IP result of anti-Calpain 6 (IP:10120-1-AP, 4ug; Detection:10120-1-AP 1:300) with human placenta tissue lysate 1520ug.

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse placenta tissue, A549 cells
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10120-1-AP targets Calpain 6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 6 fusion protein Ag0160
• Full Name: calpain 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 66-75 kDa
• GenBank Accession Number: BC000730
• Gene Symbol: CAPN6
• Gene ID (NCBI): 827
• RRID: AB_2290829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IF	J Neuropathol Exp Neurol; MicroRNA Alteration in Developing Rat Oligodendrocyte Precursor Cells Induced by Hypoxia-Ischemia.; Authors - Xiaojuan Su
human	WB,IHC	Am J Reprod Immunol; The investigation of calpain in human placenta with fetal growth restriction.; Authors - Shinobu Goto
human	WB	Amino Acids; Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6; Authors - Yufan Zhang