CAPNS1 Polyclonal antibody

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• KD/KO Validated

CAPNS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25057-1-AP Copied!

Publications (7)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CAPN4, CAPNS, CANP small subunit, Calpain small subunit 1, Calpain regulatory subunit

• 

  WB analysis of HEK-293 using 25057-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25057-1-AP (CAPNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 25057-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25057-1-AP (CAPNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 25057-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25057-1-AP (CAPNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 25057-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25057-1-AP (CAPNS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 25057-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25057-1-AP (CAPNS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 25057-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25057-1-AP (CAPNS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse spleen tissue
• Positive IHC detected in: human kidney tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25057-1-AP targets CAPNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21885

  Product name: Recombinant human CAPNS1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-323 aa of BC011903

  Sequence: MFLVNSFLKGGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEVRTPILGYGCLGGPHPSALHTSSELQSPSSYFASRPWVRAKGLVLLGFPVLTLHPPLPSGCS

• Full Name: calpain, small subunit 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC011903
• Gene Symbol: CAPNS1
• Gene ID (NCBI): 826
• RRID: AB_2879876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04632
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CAPNS1 Polyclonal antibody (Cat# 25057-1-AP) has 7 citations across journals including Cell Metabolism, Cell Reports, Biomedical Pharmacotherapy, Virulence, Oncology Reports, Journal of Biological Chemistry, and International Journal of Clinical and Experimental Pathology. Research fields span hepatocellular carcinoma metabolism, viral replication, macrophage differentiation, cGAS-STING signaling, epithelial-mesenchymal transition in melanoma, protein processing, and esophageal cancer metastasis.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu
human	WB	Biomed Pharmacother; Cereblon mediates macrophage differentiation and microglial phagocytosis by regulating calpain protease activity; Authors - Liang Zhou
		Virulence; Porcine cGAS-STING signalling induced apoptosis negatively regulates STING downstream IFN response and autophagy via different mechanisms; Authors - Nengwen Xia
		Oncol Rep; Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/β-catenin pathway.; Authors - Enwen Wang
human	WB	J Biol Chem; Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.; Authors - Hui-Yan Lei ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product reports a 5-star rating. The user tested it on mouse heart tissue by Western Blot and noted that CAPNS1 is highly expressed in mouse heart, producing a sharp, correctly sized band in heart lysates. Overall, the feedback is positive, indicating clear and specific detection in the tested tissue.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Wei (Verified Customer) (03-12-2020)

CAPNS1 is high expression in mouse heart. It has sharp, correct size band in heart lysates.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart