Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17174-1-AP targets CAPSL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10771 Product name: Recombinant human CAPSL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC017586 Sequence: MAIQAKKKLTTATNPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL Predict reactive species |
| Full Name | calcyphosine-like |
| Calculated Molecular Weight | 198 aa, 23 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC017586 |
| Gene Symbol | CAPSL |
| Gene ID (NCBI) | 133690 |
| RRID | AB_2070031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWF8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAPSL antibody 17174-1-AP | Download protocol |
| IP protocol for CAPSL antibody 17174-1-AP | Download protocol |
| WB protocol for CAPSL antibody 17174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAPSL Polyclonal antibody (Cat# 17174-1-AP) has 2 citations. One appears in Scientific Reports (2019), studying CAPSL regulation in Multiple Symmetric Lipomatosis and its role in adipogenesis using WB in mouse. The other is in Science (2026), investigating the in situ structure of the human ciliary transition zone and linking linker defects to primary ciliary dyskinesia. The associated research fields span adipose biology and ciliary/structural cell biology.
| Species | Application | Title |
|---|---|---|
Sci Rep Calcyphosine-like (CAPSL) is regulated in Multiple Symmetric Lipomatosis and is involved in Adipogenesis. | ||
Science In situ structure of the human ciliary transition zone links linker defects to primary ciliary dyskinesia. |









