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  • KD/KO Validated

CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11806-1-AP

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1



Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11806-1-AP targets CAPZA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPZA1 fusion protein Ag2267
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000144
Gene symbol CAPZA1
Gene ID (NCBI) 829
RRIDAB_2070037
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Protocols

Product Specific Protocols
WB protocol for CAPZA1 antibody 11806-1-APDownload protocol
IHC protocol for CAPZA1 antibody 11806-1-APDownload protocol
IP protocol for CAPZA1 antibody 11806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

J Cell Biol

Heterodimeric capping protein is required for stereocilia length and width regulation.

Authors - Matthew R Avenarius
humanWB,IHC

Cell Mol Life Sci

HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

Authors - Gemoll Timo T
humanWB

J Exp Clin Cancer Res

CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.

Authors - Deng Huang
  • KD Validated
humanIHC

Invest Ophthalmol Vis Sci

Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.

Authors - Linge Annett A
humanWB,IHC

Int J Oncol

Prognostic value of CAPZA1 overexpression in gastric cancer.

Authors - Lee Young-Joon YJ
  • KD Validated
humanWB,IHC

Melanoma Res

Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.

Authors - Sun Daxin D