|Positive WB detected in||human brain tissue|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human ovary tumor tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11806-1-AP targets CAPZA1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CAPZA1 fusion protein Ag2267|
|Full Name||capping protein (actin filament) muscle Z-line, alpha 1|
|Calculated molecular weight||286 aa, 33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC000144|
|Gene ID (NCBI)||829|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)
Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.
Int J Oncol
Prognostic value of CAPZA1 overexpression in gastric cancer.
Invest Ophthalmol Vis Sci
Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.
J Exp Clin Cancer Res
CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.
Cell Mol Life Sci
HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.
J Cell Biol
Heterodimeric capping protein is required for stereocilia length and width regulation.